ProPCR HotStart Taq Mix

The Proteogenixx ProPCR HotStart Taq Mix is a high-performance 2X master mix formulated for precise and efficient DNA amplification.

Description

The Proteogenixx ProPCR HotStart Taq Mix is a high-performance 2X master mix formulated for precise and efficient DNA amplification. Incorporating antibody-mediated HotStart Taq DNA Polymerase, the enzyme remains inactive at ambient temperature, preventing non-specific amplification and primer-dimer formation during reaction setup. Activation occurs during the initial denaturation step, ensuring high specificity and robust amplification.

This optimized master mix contains Taq DNA Polymerase, reaction buffer, MgCl₂, and dNTPs at ideal concentrations, enabling streamlined PCR setup with reduced pipetting steps and enhanced reproducibility. The formulation is suitable for amplification of genomic DNA, plasmid DNA, and cDNA templates across a broad range of applications in molecular biology research.

Storage: Store at –20 °C. Stable for up to 24 months when stored under recommended conditions. Avoid repeated freeze-thaw cycles.

Specification Details
Product Type Hot Start PCR Mix
Pack Size 1 mL
Overhang 3′-A Overhang
Polymerase Taq DNA Polymerase
Storage Store at –20 °C for long-term stability
Reaction Format SuperMix / Master Mix
Concentration 2X
For Use With (Application) Hot-Start PCR
Reaction Speed Standard
Unit Size Each
  • HotStart Technology: Minimizes non-specific amplification and improves reaction specificity
  • 2X Ready-to-Use Master Mix: Simplifies reaction preparation and reduces handling errors
  • High Sensitivity & Yield: Efficient amplification from low-copy templates
  • Reproducible Performance: Consistent results across experiments
  • Optimized Formulation: Contains Taq polymerase, dNTPs, MgCl₂, and reaction buffer
  • Applications: Ideal for routine PCR, colony PCR, cloning, genotyping, and research-based molecular diagnostics

Precautions and Disclaimer

For research and development use only. Not intended for use in diagnostics, therapeutics, or household applications.

Key Documents

• SDS (Safety Data Sheet)
• COA (Certificate of Analysis)