Phos-tag™ Agarose Resins in Tip

Phos-tag^™ Tip is a ready-to-use pipette-tip column prepacked with Phos-tag^™ agarose. This column is a powerful tool for pretreating phosphorylated peptides.

Description

Phos-tag^™ Tip is a ready-to-use pipette-tip column prepacked with Phos-tag^™ agarose. This column is a powerful tool for pretreating phosphorylated peptides.
The Phos-tag^™ molecules in a pipette tip can specifically capture phosphorylated proteins in your samples. This allows for the easy purification of these proteins using only a pipetting operation.

Product Information

Instructions for Use

Features

Short hands-on time (less than 30 min)
High recovery yields
No need for expensive equipment

Separation of Phosphopeptides from the Tryptic Digest of 6 nM β-Casein

Methods

Following trypsin digestion of β-casein (6 nM), phosphorylated peptides were purified using a Phos-tag^™ Tip column.
Aliquots of the unpurified sample, flow-through fraction, and eluate were analyzed by HPLC.

Results

We detected only phosphopeptide peaks (P1 and P2) in the eluate. This indicates that Phos-tag^™ Tips can selectively trap and enrich phosphopeptides from the tryptic digest of β-casein.

P1: FQpSEEQQQTERELQDK
P2: RELEELDVPGEIVEpSLpSpSpSEESITR