HEPES
HEPES
HEPES

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a widely used zwitterionic buffering agent from the Good’s buffer family, valued for its excellent buffering capacity near

Description

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a widely used zwitterionic buffering agent from the Good’s buffer family, valued for its excellent buffering capacity near physiological pH. With an effective buffering range of pH 6.8–8.2, HEPES is extensively employed in molecular biology, biochemistry, protein chemistry, and cell culture applications where precise pH control is critical.

Proteogenixx’s HEPES Buffer Grade is manufactured to high purity standards, ensuring minimal interference with enzymatic reactions and biological systems. Its strong buffering capacity, low metal ion binding, and chemical stability make it ideal for long-duration experiments, including cell-based assays and protein studies. HEPES is particularly advantageous in CO₂-independent systems, maintaining stable pH even outside incubator environments.

Storage: Store tightly sealed at room temperature in a dry place to ensure long-term stability.

SPECIFICATION
Specification Description
Product Type HEPES
Format High-purity, crystalline buffering reagent
Chemical Name 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
Application Preparation of biological and biochemical buffers; widely used in cell culture media, protein purification, enzyme assays, and molecular biology workflows requiring stable physiological pH
Compatible Equipment Standard laboratory buffer preparation systems, pH meters, cell culture setups, and biochemical assay platforms
Purity Level ≥ 99% (Molecular Biology Grade); DNase-/RNase-free
pKa Value 7.55 at 25 °C
pH Range Effective buffering capacity between pH 6.8–8.2
Pack Sizes 500 g, 1 kg, 2.5 kg
Solubility Freely soluble in water, ensuring precise and reproducible buffer preparation
Storage Store tightly sealed at room temperature; protect from moisture and light
Unit Size Each container supplied as high-quality, free-flowing crystalline powder suitable for sensitive molecular and cell-based applications

Additional information

Catalog No.

PX-BF-201-100G, PX-BF-201-1KG, PX-BF-201-250G, PX-BF-201-25G, PX-BF-201-500G
Pack Size

1 KG, 100 G, 25 G, 250 G, 500 G

Key Advantages

Physiological pH Buffering – Effective buffering range of pH 6.8–8.2, ideal for biological systems.
High Purity Grade – Suitable for sensitive molecular biology and cell culture applications.
Excellent pH Stability – Maintains consistent pH during extended experimental procedures.
Low Metal Ion Interaction – Preserves enzyme activity and protein integrity.
CO₂-Independent Buffering – Ideal for cell culture work outside CO₂ incubators.
Low UV Absorbance – Compatible with spectrophotometric and analytical assays.
Versatile Applications – Used in protein purification, enzyme assays, electrophoresis buffers, and cell culture media.

Precautions and Disclaimer

For research and development use only. This product is not intended for drug, household, or any other unauthorized applications.

Key Documents

  • SDS (Safety Data Sheet)
  • COA (Certificate of Analysis)

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