CBB Colloidal Staining Solution

Coomassie Brilliant Blue (CBB) staining is one of the most widely adopted techniques for protein detection in polyacrylamide gels, particularly SDS-PAGE.

Description

Coomassie Brilliant Blue (CBB) staining is one of the most widely adopted techniques for protein detection in polyacrylamide gels, particularly SDS-PAGE. Known for its simplicity, efficiency, and affordability, CBB staining remains a staple method in molecular biology and biochemistry laboratories.

The dye interacts primarily with basic amino acids such as arginine, lysine, and histidine, forming stable dye–protein complexes that appear as distinct blue protein bands against a clear gel background. The method allows reliable detection of proteins at levels as low as 30–100 ng, depending on the formulation.

The CBB Ultra formulation enhances this classical method by offering ultra-sensitive detection with a simplified workflow. It eliminates the need for gel fixing and destaining steps—protein bands appear within 5–10 minutes after rinsing with water, providing rapid and clear visualization with minimal background. Importantly, the stain is also fully compatible with mass spectrometry-based protein identification, making it suitable for proteomic workflows.

This kit is supplied with following components:

Component Catalog No. Storage
CBB Staining Solution PX-CCBB-301 Room Temperature
SPECIFICATION
Specification Description
Assay Protein staining in SDS-PAGE gels
Concentration Ready-to-use solution
For Use With (Application) Visualization of proteins in polyacrylamide gels
For Use With (Equipment) Gel electrophoresis apparatus, imaging systems
Kit / Buffer Contents CBB Colloidal Staining Solution (ultra-sensitive Coomassie-based stain)
Product Type Protein Visualization Reagent
Quantity 500 mL& 1L
Sufficient For Multiple SDS-PAGE gel staining runs
Detection Method Colorimetric (blue protein bands on gel background)
Unit Size Each
Storage Store at 2–8 °C

Additional information

Catalog No.

PX-CCBB-301-1L, PX-CCBB-301-500ml

Pack Size

1 L, 500 mL

Key Features & Advantages

Detection Sensitivity – 30–100 ng of protein
Fast Protocol – Clear bands in 5–10 minutes.
Workflow – No fixing or destaining required.
Compatibility – Mass spectrometry–friendly.
Applications – Routine SDS-PAGE protein detection and proteomics.
Cost-Effective – Simple and economical solution for everyday protein analysis.

Precautions and Disclaimer

For research and development use only. This product is not intended for drug, household, or any other unauthorized applications.

Key Documents

  • SDS (Safety Data Sheet)
  • COA (Certificate of Analysis)