Bradford Assay Kit
Bradford Assay Kit
Bradford Assay Kit

Accurate determination of protein concentration is fundamental to biochemical and molecular biology research. The Bradford assay, first described by Marion M. Bradford, has become one of the most widely used methods due to its speed, sensitivity, and simplicity compared to the Lowry assay.

Description

Accurate determination of protein concentration is fundamental to biochemical and molecular biology research. The Bradford assay, first described by Marion M. Bradford, has become one of the most widely used methods due to its speed, sensitivity, and simplicity compared to the Lowry assay. Its reduced susceptibility to interference from many non-protein components further enhances its reliability in routine laboratory applications.

The Bradford method relies on the binding of Coomassie Brilliant Blue G-250 dye to proteins. In acidic conditions, the dye exists in multiple ionic forms (red, green, and blue). Upon protein binding, the dye shifts predominantly to its blue anionic form, which strongly absorbs at 595 nm. The absorbance measured at this wavelength is directly proportional to protein concentration, allowing precise and reproducible quantification.

This kit is supplied with following components:

Component Catalog No. Storage
Bradford Reagent PX-BDR-201 Room Temperature
Protein Standard PX-PS-101 2-8℃
SPECIFICATION
Parameter / Attribute Details
Assay Type Bradford Protein Assay
Standard Concentration Bovine Serum Albumin (BSA): 2 mg/mL
Detection Method Colorimetric (Absorbance at 595 nm)
Application Protein Quantification in Solution
Compatible Equipment Spectrophotometer, Microplate Reader
Kit Contents Dye Reagent Concentrate (sufficient for dilutions) BSA Standard (Lyophilized or solution form)
Specificity General protein detection; not target-specific
Sufficient For ~250 cuvette assays and ~500 cuvette assays
Product Format Ready-to-use reagent with dilution flexibility
Unit Size / Quantity 500mL and 1 L capacity

Additional information

Catalog No.

PX-PROTQ-201-1L, PX-PROTQ-201-500ML
Pack Size

1 L, 500 mL

Key Features & Advantages

Fast & Sensitive – Delivers accurate protein measurements within minutes.

Reduced Interference – Less affected by many non-protein components compared to the Lowry assay.

High Specificity – Color shift (to blue form) occurs only upon protein binding, enabling robust quantification.

Compatible with Reducing Agents – Unlike Lowry and BCA assays, effective in the presence of reducing agents.

Cost-Effective & Convenient – Simple, single-step protocol with minimal reagent preparation.

Precautions and Disclaimer

For research and development use only. This product is not intended for drug, household, or any other unauthorized applications

Key Documents

  • SDS (Safety Data Sheet)
  • COA (Certificate of Analysis)

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