BCA Estimation Kit with Protein Standard
BCA Estimation Kit with Protein Standard
BCA Estimation Kit with Protein Standard
Accurate protein quantification is essential in life science research, from enzyme activity studies and protein–protein interaction assays to sample preparation for advanced downstream analyses.

Description

Accurate protein quantification is essential in life science research, from enzyme activity studies and protein–protein interaction assays to sample preparation for advanced downstream analyses. The BCA Protein Estimation Kit provides a dependable solution, delivering precise, reproducible results across a wide range of biological samples, including cell lysates, serum, plasma, and urine.

Using the well-established bicinchoninic acid (BCA) chemistry, the assay produces a stable purple color when protein–copper complexes are formed under alkaline conditions. The intensity of this color, measurable at 562 nm, directly correlates with protein concentration, ensuring consistent and quantitative assessment.

This kit is supplied with following components:

Reagent Catalog No. Storage
A PX-RGA-101 Room Temperature
B PX-RGB-101
Protein Standard PX-PS-101 2-8℃
SPECIFICATION
Specification Description
Method BCA (Bicinchoninic Acid) Protein Assay
Reference Protein BSA (Bovine Serum Albumin), 2 mg/mL
Intended Use Solution-based protein detection, absorbance measurement
Instruments Spectrophotometer, Microplate Reader
Components 10 × 1 mL glass ampoules
Category Protein Quantitation Assay
Pack Size 1 Kit (250 ml) and (500ml)
Target Range General protein quantification (not target-specific)
Throughput ~250 cuvette assays and ~500 cuvette assays
Readout Colorimetric (absorbance at 562 nm)
Packaging Unit Each

Additional information

Catalog No.

PX-PROTQ-101-250ML, PX-PROTQ-101-500ML
Pack Size

250 mL, 500 mL

Key Advantages

Broad Compatibility – Works effectively in the presence of ionic and non-ionic detergents, making it ideal for lysates and extraction buffers.

High Accuracy – Less affected by protein-to-protein variation than dye-binding assays such as Bradford.

Convenient & Efficient – Simple protocol with minimal hands-on time; results in under 30 minutes.

Flexible Format – Sufficient for ~2,500 microplate reactions or 500 cuvette assays.

Precautions and Disclaimer

For research and development use only. This product is not intended for drug, household, or any other unauthorized applications

Key Documents

  • SDS (Safety Data Sheet)
  • COA (Certificate of Analysis)

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