NanoShield™ Trap Columns
NanoShield™ Trap Columns
NanoShield™ Trap Columns

NanoShield™ 5×100 NZ C18 trap column 3-Pack

5 cm x 100 μm C18 UHPLC trap column with 2 x nanoZero® fittings compatible with nanoViper™

SPD 10 – 300
Gradients Use standard Aurora Series® analytical gradients for elution
Mode Reversed-flush

Description

Chromatographic trap columns are essential in proteomic UHPLC–MS workflows, improving analyte focusing and enabling rapid sample loading with in-line sample clean-up to protect analytical columns from blockages and contamination while reducing ion suppression. These benefits extend column lifetime and reduce maintenance, supporting robust, high-throughput, and reproducible proteomic analyses. Despite these advantages, trap columns traditionally sacrifice peptide and protein identifications compared to direct injection. The NanoShield™ C18 5 cm × 100 μm trap column eliminates this trade-off – delivering direct injection-level performance; providing superior peptide identifications, peak quality, and hydrophilic peptide coverage compared to other traps.

Product benefits Ideal for
1. Next to no peptide ID loss

2. Extends column life, reduces maintenance costs

3. Improved reproducibility across thousands of injections

4. Enables hundreds of samples per day

 1. Fast sample loading

2. Reversed-flush workflows

3. Bottom-up discovery workflows

A perfectly paired trap for Aurora Series columns.

A 100 ng HeLa tryptic digest was analysed using a reverse-flush trap-elute method compared to direct injection across two workflows: a 37-minute gradient on the Aurora® Ultimate™ CSI (25 cm x 75 μm) column and a 14-minute gradient on the Aurora® Rapid™ CSI (8 cm × 75 μm) column, both analysed on a Bruker nanoElute 2 UHPLC coupled to a Bruker timsTOF HT mass spectrometer.

The NanoShield™ trap column (5 cm x 100 μm, 3 μm C18 particles) demonstrated near-direct injection performance across all metrics while substantially outperforming a competitor C18 trap cartridge (5 mm x 300 μm, 5 μm C18 particles). NanoShield™ showed less than 1% reduction in protein identifications and a 1–3% reduction in precursor identifications compared to direct injection across both columns.

Peak quality metrics remained similarly robust, with marginal increases in FWHM and minimal changes to peak shape ratios compared to direct injection, while maintaining significantly superior performance relative to the competitor trap.

Specification Description
Column format Trap/guard column
Column type Reversed-phase
Trap column orientation Reverse flush
For use with UHPLC
Length 5 cm
Inner diameter 100 µm
Pore size 120 Å
Pressure rating >1700 bar
Particle size 3 µm
pH stability 1–8
Stationary phase C18
Suggested loading capacity 200 ng
Max loading capacity 1 µg
Suggested loading pressure < 1000 bar

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